Simultaneously with the work of American authors, the search for the “substance of sleep” took place in Europe. But they were completely independent: ex sharply and chalis and theoretical assumptions, and methods. That turned out to be a horse and h nye results. Swiss scientist M. Monnier and G. Shonenbergeru to allocate 0.3 mg “sleeping agents” of the venous blood 14 rabbits, conceded Mr. Noah through the apparatus “artificial kidney”. Previously these rabbits by d jected session “electric sleep” stimulation by weak current definition of n various points of the brain in depth, open-40-ies. outstanding Swiss physiologist, Nobel laureate V. Hess. The substance proved Neboli s PWM previously unknown peptide which was named “peptide causing th conductive delta sleep» (Delta Sleep-Inducing Peptide, DSIP) due to its ability to, according discoverer, increase the deepest stage IU q lennogo sleep. We conducted a study of this substance in experiments on rabbits and rats and could not confirm its “hypnotic” properties.
To the same p e Other researchers from Switzerland, the United States, Hungary and other countries also came. The situation seemed paradoxical, but we seem to have managed to find ways to resolve it. In biochemical studies, it was revealed that the DSIP peptide is a highly unstable substance and when introduced into the body after a few minutes, is destroyed by the action of enzymes. We decided to check what would happen if, instead of DSIP itself, it was introduced from the structural chemical derivatives, the so-called “structural analogs of gi”, the structure of which is very similar to the structure of the DSIP itself, but is stable . The visibility is much higher. A large group of such analogues was synthesized in two domestic laboratories — I. I. Mikhaleva and co-authors at the same Institute of Bioorganic Chemistry, as well as V. N. Kalichevich and S. I. Churkina at the Institute of Chemistry at St. Petersburg University. We have studied their effects on sleep guinea pigs and white rats at Various hours GOVERNMENTAL doses and methods of administration, and received a number of new and unexpected p f the results. It turned out that, modifying the DSIP molecule, one can observe both an increase and a decrease in sleep in animals, and the severity, nature and dynamics of the effect depend on the structure of the introduced peptide. N a For example, some peptides have a greater effect on slow sleep, others on a paradoxical one, and still others on both phases. In some, the maximum effect falls on the period immediately following the administration of the drug, while in others this peak is delayed by several hours.
The variety of effects is so large that can create a striking impression as if a complicated function of the organism, which is a natural sleep, which involved in the regulation, probably thousands of different biochemical substances can be controlled by changing the structure of a single original Molek in ly! Mechanisms of action of the peptide to sleep DSIP and its analogues so far little of in cheny. However, it is known that this peptide plays an important role in the endocrine regulation of the organism, suppressing the release of stress hormones and stimulating p mony growth. Since both those, and others are involved in the regulation of sleep, it is possible that DSIP effect on sleep not only directly, but also indirectly called nym way through the hormonal system, with which it is associated. We are s said speculation that DSIP peptide – is the first representative of a previously unknown class of regulators “high level”, carrying koo p -ordination among the various systems of the body.